AgrobacTransV1, Main, Exploration, bibRecord, 000684

Expression of HIV-1 antigens in plants as potential subunit vaccines.

Identifieur interne : 000684 ( Main/Exploration ); précédent : 000683; suivant : 000685

Expression of HIV-1 antigens in plants as potential subunit vaccines.

Auteurs : Ann Meyers [Afrique du Sud] ; Ereck Chakauya ; Enid Shephard ; Fiona L. Tanzer ; James Maclean ; Alisson Lynch ; Anna-Lise Williamson ; Edward P. Rybicki

Source :

BMC biotechnology [ 1472-6750 ] ; 2008.

RBID : pubmed:18573204

Descripteurs français

English descriptors

KwdEn :
- AIDS Vaccines (biosynthesis), AIDS Vaccines (genetics), Adjuvants, Immunologic (genetics), Agrobacterium tumefaciens (genetics), Animals (MeSH), Chloroplasts (genetics), Chloroplasts (metabolism), Endoplasmic Reticulum (genetics), Endoplasmic Reticulum (metabolism), Female (MeSH), Gene Expression (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, gag (MeSH), Genetic Vectors (MeSH), HIV Antigens (biosynthesis), HIV Antigens (genetics), HIV Antigens (immunology), HIV Infections (immunology), HIV Seronegativity (MeSH), HIV-1 (genetics), Humans (MeSH), Mice (MeSH), Mice, Inbred BALB C (MeSH), Plants, Genetically Modified (MeSH), Tobacco (genetics), Tobamovirus (genetics), Transformation, Genetic (MeSH), Vaccines, Subunit (biosynthesis), Vaccines, Subunit (genetics), gag Gene Products, Human Immunodeficiency Virus (biosynthesis), gag Gene Products, Human Immunodeficiency Virus (genetics).
MESH :
- chemical , biosynthesis : AIDS Vaccines, HIV Antigens, Vaccines, Subunit, gag Gene Products, Human Immunodeficiency Virus.
- chemical , genetics : AIDS Vaccines, Adjuvants, Immunologic, HIV Antigens, Vaccines, Subunit, gag Gene Products, Human Immunodeficiency Virus.
- genetics : Agrobacterium tumefaciens, Chloroplasts, Endoplasmic Reticulum, HIV-1, Tobacco, Tobamovirus.
- chemical , immunology : HIV Antigens, HIV Infections.
- metabolism : Chloroplasts, Endoplasmic Reticulum.
- Animals, Female, Gene Expression, Gene Expression Regulation, Plant, Genes, gag, Genetic Vectors, HIV Seronegativity, Humans, Mice, Mice, Inbred BALB C, Plants, Genetically Modified, Transformation, Genetic.

Abstract

BACKGROUND

Human immunodeficiency virus type 1 (HIV-1) has infected more than 40 million people worldwide, mainly in sub-Saharan Africa. The high prevalence of HIV-1 subtype C in southern Africa necessitates the development of cheap, effective vaccines. One means of production is the use of plants, for which a number of different techniques have been successfully developed. HIV-1 Pr55Gag is a promising HIV-1 vaccine candidate: we compared the expression of this and a truncated Gag (p17/p24) and the p24 capsid subunit in Nicotiana spp. using transgenic plants and transient expression via Agrobacterium tumefaciens and recombinant tobamovirus vectors. We also investigated the influence of subcellular localisation of recombinant protein to the chloroplast and the endoplasmic reticulum (ER) on protein yield. We partially purified a selected vaccine candidate and tested its stimulation of a humoral and cellular immune response in mice.

RESULTS

Both transient and transgenic expression of the HIV antigens were successful, although expression of Pr55Gag was low in all systems; however, the Agrobacterium-mediated transient expression of p24 and p17/p24 yielded best, to more than 1 mg p24/kg fresh weight. Chloroplast targeted protein levels were highest in transient and transgenic expression of p24 and p17/p24. The transiently-expressed p17/p24 was not immunogenic in mice as a homologous vaccine, but it significantly boosted a humoral and T cell immune response primed by a gag DNA vaccine, pTHGagC.

CONCLUSION

Transient agroinfiltration was best for expression of all of the recombinant proteins tested, and p24 and p17/p24 were expressed at much higher levels than Pr55Gag. Our results highlight the usefulness of plastid signal peptides in enhancing the production of recombinant proteins meant for use as vaccines. The p17/p24 protein effectively boosted T cell and humoral responses in mice primed by the DNA vaccine pTHGagC, showing that this plant-produced protein has potential for use as a vaccine.

DOI: 10.1186/1472-6750-8-53
PubMed: 18573204
PubMed Central: PMC2443125

Affiliations:

Afrique du Sud

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000675
to stream Main, to step Curation: 000675

Le document en format XML

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<term>AIDS Vaccines (genetics)</term>
<term>Adjuvants, Immunologic (genetics)</term>
<term>Agrobacterium tumefaciens (genetics)</term>
<term>Animals (MeSH)</term>
<term>Chloroplasts (genetics)</term>
<term>Chloroplasts (metabolism)</term>
<term>Endoplasmic Reticulum (genetics)</term>
<term>Endoplasmic Reticulum (metabolism)</term>
<term>Female (MeSH)</term>
<term>Gene Expression (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genes, gag (MeSH)</term>
<term>Genetic Vectors (MeSH)</term>
<term>HIV Antigens (biosynthesis)</term>
<term>HIV Antigens (genetics)</term>
<term>HIV Antigens (immunology)</term>
<term>HIV Infections (immunology)</term>
<term>HIV Seronegativity (MeSH)</term>
<term>HIV-1 (genetics)</term>
<term>Humans (MeSH)</term>
<term>Mice (MeSH)</term>
<term>Mice, Inbred BALB C (MeSH)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Tobacco (genetics)</term>
<term>Tobamovirus (genetics)</term>
<term>Transformation, Genetic (MeSH)</term>
<term>Vaccines, Subunit (biosynthesis)</term>
<term>Vaccines, Subunit (genetics)</term>
<term>gag Gene Products, Human Immunodeficiency Virus (biosynthesis)</term>
<term>gag Gene Products, Human Immunodeficiency Virus (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adjuvants immunologiques (génétique)</term>
<term>Agrobacterium tumefaciens (génétique)</term>
<term>Animaux (MeSH)</term>
<term>Antigènes du VIH (biosynthèse)</term>
<term>Antigènes du VIH (génétique)</term>
<term>Antigènes du VIH (immunologie)</term>
<term>Chloroplastes (génétique)</term>
<term>Chloroplastes (métabolisme)</term>
<term>Expression des gènes (MeSH)</term>
<term>Femelle (MeSH)</term>
<term>Gènes gag (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Infections à VIH (immunologie)</term>
<term>Produits du gène gag du virus de l'immunodéficience humaine (biosynthèse)</term>
<term>Produits du gène gag du virus de l'immunodéficience humaine (génétique)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Réticulum endoplasmique (génétique)</term>
<term>Réticulum endoplasmique (métabolisme)</term>
<term>Souris (MeSH)</term>
<term>Souris de lignée BALB C (MeSH)</term>
<term>Séronégativité VIH (MeSH)</term>
<term>Tabac (génétique)</term>
<term>Tobamovirus (génétique)</term>
<term>Transformation génétique (MeSH)</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique)</term>
<term>Vaccins contre le SIDA (biosynthèse)</term>
<term>Vaccins contre le SIDA (génétique)</term>
<term>Vaccins sous-unitaires (biosynthèse)</term>
<term>Vaccins sous-unitaires (génétique)</term>
<term>Vecteurs génétiques (MeSH)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>AIDS Vaccines</term>
<term>HIV Antigens</term>
<term>Vaccines, Subunit</term>
<term>gag Gene Products, Human Immunodeficiency Virus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>AIDS Vaccines</term>
<term>Adjuvants, Immunologic</term>
<term>HIV Antigens</term>
<term>Vaccines, Subunit</term>
<term>gag Gene Products, Human Immunodeficiency Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Antigènes du VIH</term>
<term>Produits du gène gag du virus de l'immunodéficience humaine</term>
<term>Vaccins contre le SIDA</term>
<term>Vaccins sous-unitaires</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term>
<term>Chloroplasts</term>
<term>Endoplasmic Reticulum</term>
<term>HIV-1</term>
<term>Tobacco</term>
<term>Tobamovirus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Adjuvants immunologiques</term>
<term>Agrobacterium tumefaciens</term>
<term>Antigènes du VIH</term>
<term>Chloroplastes</term>
<term>Produits du gène gag du virus de l'immunodéficience humaine</term>
<term>Réticulum endoplasmique</term>
<term>Tabac</term>
<term>Tobamovirus</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term>
<term>Vaccins contre le SIDA</term>
<term>Vaccins sous-unitaires</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes du VIH</term>
<term>Infections à VIH</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>HIV Antigens</term>
<term>HIV Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Chloroplasts</term>
<term>Endoplasmic Reticulum</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Chloroplastes</term>
<term>Réticulum endoplasmique</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Female</term>
<term>Gene Expression</term>
<term>Gene Expression Regulation, Plant</term>
<term>Genes, gag</term>
<term>Genetic Vectors</term>
<term>HIV Seronegativity</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Plants, Genetically Modified</term>
<term>Transformation, Genetic</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Expression des gènes</term>
<term>Femelle</term>
<term>Gènes gag</term>
<term>Humains</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Séronégativité VIH</term>
<term>Transformation génétique</term>
<term>Vecteurs génétiques</term>
<term>Végétaux génétiquement modifiés</term>
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<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>Human immunodeficiency virus type 1 (HIV-1) has infected more than 40 million people worldwide, mainly in sub-Saharan Africa. The high prevalence of HIV-1 subtype C in southern Africa necessitates the development of cheap, effective vaccines. One means of production is the use of plants, for which a number of different techniques have been successfully developed. HIV-1 Pr55Gag is a promising HIV-1 vaccine candidate: we compared the expression of this and a truncated Gag (p17/p24) and the p24 capsid subunit in Nicotiana spp. using transgenic plants and transient expression via Agrobacterium tumefaciens and recombinant tobamovirus vectors. We also investigated the influence of subcellular localisation of recombinant protein to the chloroplast and the endoplasmic reticulum (ER) on protein yield. We partially purified a selected vaccine candidate and tested its stimulation of a humoral and cellular immune response in mice.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>Both transient and transgenic expression of the HIV antigens were successful, although expression of Pr55Gag was low in all systems; however, the Agrobacterium-mediated transient expression of p24 and p17/p24 yielded best, to more than 1 mg p24/kg fresh weight. Chloroplast targeted protein levels were highest in transient and transgenic expression of p24 and p17/p24. The transiently-expressed p17/p24 was not immunogenic in mice as a homologous vaccine, but it significantly boosted a humoral and T cell immune response primed by a gag DNA vaccine, pTHGagC.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSION</b>
</p>
<p>Transient agroinfiltration was best for expression of all of the recombinant proteins tested, and p24 and p17/p24 were expressed at much higher levels than Pr55Gag. Our results highlight the usefulness of plastid signal peptides in enhancing the production of recombinant proteins meant for use as vaccines. The p17/p24 protein effectively boosted T cell and humoral responses in mice primed by the DNA vaccine pTHGagC, showing that this plant-produced protein has potential for use as a vaccine.</p>
</div>
</front>
</TEI>
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<DateCompleted><Year>2008</Year>
<Month>09</Month>
<Day>02</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1472-6750</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>8</Volume>
<PubDate><Year>2008</Year>
<Month>Jun</Month>
<Day>23</Day>
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<Title>BMC biotechnology</Title>
<ISOAbbreviation>BMC Biotechnol</ISOAbbreviation>
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<ArticleTitle>Expression of HIV-1 antigens in plants as potential subunit vaccines.</ArticleTitle>
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<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Human immunodeficiency virus type 1 (HIV-1) has infected more than 40 million people worldwide, mainly in sub-Saharan Africa. The high prevalence of HIV-1 subtype C in southern Africa necessitates the development of cheap, effective vaccines. One means of production is the use of plants, for which a number of different techniques have been successfully developed. HIV-1 Pr55Gag is a promising HIV-1 vaccine candidate: we compared the expression of this and a truncated Gag (p17/p24) and the p24 capsid subunit in Nicotiana spp. using transgenic plants and transient expression via Agrobacterium tumefaciens and recombinant tobamovirus vectors. We also investigated the influence of subcellular localisation of recombinant protein to the chloroplast and the endoplasmic reticulum (ER) on protein yield. We partially purified a selected vaccine candidate and tested its stimulation of a humoral and cellular immune response in mice.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Both transient and transgenic expression of the HIV antigens were successful, although expression of Pr55Gag was low in all systems; however, the Agrobacterium-mediated transient expression of p24 and p17/p24 yielded best, to more than 1 mg p24/kg fresh weight. Chloroplast targeted protein levels were highest in transient and transgenic expression of p24 and p17/p24. The transiently-expressed p17/p24 was not immunogenic in mice as a homologous vaccine, but it significantly boosted a humoral and T cell immune response primed by a gag DNA vaccine, pTHGagC.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Transient agroinfiltration was best for expression of all of the recombinant proteins tested, and p24 and p17/p24 were expressed at much higher levels than Pr55Gag. Our results highlight the usefulness of plastid signal peptides in enhancing the production of recombinant proteins meant for use as vaccines. The p17/p24 protein effectively boosted T cell and humoral responses in mice primed by the DNA vaccine pTHGagC, showing that this plant-produced protein has potential for use as a vaccine.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Meyers</LastName>
<ForeName>Ann</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Observatory 7925, South Africa. ann.meyers@uct.ac.za</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Chakauya</LastName>
<ForeName>Ereck</ForeName>
<Initials>E</Initials>
</Author>
<Author ValidYN="Y"><LastName>Shephard</LastName>
<ForeName>Enid</ForeName>
<Initials>E</Initials>
</Author>
<Author ValidYN="Y"><LastName>Tanzer</LastName>
<ForeName>Fiona L</ForeName>
<Initials>FL</Initials>
</Author>
<Author ValidYN="Y"><LastName>Maclean</LastName>
<ForeName>James</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="Y"><LastName>Lynch</LastName>
<ForeName>Alisson</ForeName>
<Initials>A</Initials>
</Author>
<Author ValidYN="Y"><LastName>Williamson</LastName>
<ForeName>Anna-Lise</ForeName>
<Initials>AL</Initials>
</Author>
<Author ValidYN="Y"><LastName>Rybicki</LastName>
<ForeName>Edward P</ForeName>
<Initials>EP</Initials>
</Author>
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<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
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<Month>06</Month>
<Day>23</Day>
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<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
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<MeshHeading><DescriptorName UI="D002736" MajorTopicYN="N">Chloroplasts</DescriptorName>
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Expression of HIV-1 antigens in plants as potential subunit vaccines.

Expression of HIV-1 antigens in plants as potential subunit vaccines.

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